The ad will run on Biden's X, Facebook, Instagram and Threads social media accounts, the campaign said.
In an interview on Monday, CNBC host Joe Kernan asked Trump “How to deal with entitlements: Social Security, Medicare and Medicaid?”
Kernan's question was based on concerns that those programs would continue to add fuel to the U.S. national debt if they were not curtailed.
Trump responded: “There's a lot you can do in terms of entitlements — cutting edge — and theft and mismanagement of entitlements.”
The Biden campaign highlighted that response, concluding with footage from the president's State of the Union address on Thursday, “If anybody here tries to raise Social Security, Medicare or the retirement age, I'm going to stop you.”
The ad was part of a series of responses by Biden and his campaign to Trump's interview, which later took the opportunity to attack the Republican Party for recommending cuts to programs that benefit more than 150 million Americans, many of them older voters.
Biden's campaign first tweeted footage of the Trump interview and Biden's official X account, then responded with a statement saying, “Not on my watch.”
“This morning, Donald Trump said cuts to Social Security and Medicare are back on the table,” Biden said during a campaign event in New Hampshire on Monday.
“The bottom line is, he's still at it,” Biden said. “I will never allow that. I will never cut Social Security. I will never cut Medicare.”
White House Press Secretary Andrew Bates issued a separate statement highlighting the president's promise to protect the program.
Meanwhile, Trump's team tried to explain his comments on the “squawk box” on Monday.
“You know President Trump is talking about cutting waste if the losers don't tone down his response,” the Trump campaign said in a tweet in response to the Biden campaign's post featuring a video of the former president.